Effect of Adept Agro.Bio on Biochemical Processes in Plants and Yield Formation

To establish the connection between the effect of Adept Agro.Bio on substance transformation in soil and plants, biochemical studies of wheat vegetative mass were conducted.

Biochemical Processes in Wheat Vegetative Mass

Table 7. Biochemical Indicators of Wheat Vegetative Mass

(as % of dry plant material weight, June 12, 2019)

Experiment Variant Total Nitrogen Protein Amino Acids Non-protein Nitrogenous Compounds Reducing Sugars Organic Acids
Without Fertilizers 2.06 12.30 0.98 9.68 1.54 1.83
Adept 2 l/ha 2.86 17.16 1.54 12.82 2.41 1.83
Adept 2 l/ha (repeated) 2.79 16.74 2.06 13.62 2.63 2.27
25% Ammonia Water 240 l/ha 2.88 17.30 1.76 12.97 2.08 2.37

Analysis Methods

  • Quantitative determination of sugars was performed using Bertrand's method.
  • Organic acids were determined by titration.
  • Amino acids were determined by formol titration.
  • Protein content was determined using Bernstein's method.
  • Total nitrogen was determined by the Kjeldahl method.
  • Non-protein nitrogen was calculated as the difference between total nitrogen and protein nitrogen precipitated by heavy metal salts.

Results

The data show changes in the accumulation and transformation of substances in plants due to improved soil nutrition. Fertilized plants demonstrate increased content of simple carbohydrates, amino acids, and nitrogen. The most active accumulation of reducing sugars during the tillering stage of wheat was observed with the use of humic fertilizer Adept Agro.Bio. The increase in sugar content is associated with enhanced enzymatic synthesis and profound changes in metabolism.

The content of organic acids also increases in fertilized plants. The form of nitrogen nutrition affects their accumulation: physiologically acidic salts reduce the content of organic acids, while humic fertilizers do not show this effect. The increase in organic acid content correlates with the intensity of protein metabolism, as confirmed by protein accumulation data (Table 7). This correlation is likely related to the direct amination of organic acids, as the nitrogen introduced with fertilizers is actively involved in the synthesis of amino acids, proteins, and other nitrogenous compounds.

Effect on Plant Development

Humic fertilizers Adept at a dose of 2 l/ha have a pronounced effect: plants show powerful development and intense dark green coloration. With repeated application of the humic fertilizer at the same dose (Adept 2 l/ha), differences from control plants appear later - during the spike formation and grain filling stages. Wheat fertilized with humic fertilizers has thicker straw, larger spikes, and grains. The grain quality surpasses that from plots fertilized with mineral fertilizers or ammonia water.

Yield Characteristics

Table 8. Characteristics of Wheat Yield from Differently Fertilized Plots of Gray Forest Soil

Experiment Variant Spike Length, cm Number of Spikelets per Spike Number of Grains per Spike Absolute Grain Weight, g Grain Test Weight (weight of 1/4 liter of grain), g Yield, c/ha Yield Increase, c/ha Yield Increase per 1 kg of Nitrogen, kg
Without Fertilizers 6.0 9.7 21.8 31.39 177.6 14.6
Adept 2 l/ha (repeated) 6.2 10.2 22.9 31.79 177.8 17.6 3.0 15.0
Adept 2 l/ha 6.5 10.8 23.2 33.39 180.6 17.8 3.2 8.0
25% Ammonia Water 240 l/ha 6.1 10.3 20.4 32.68 176.8 16.9 2.3 4.6

Conclusions

Humic fertilizer Adept Agro.Bio activates biochemical processes in plants, promoting the accumulation of nutrients and easily hydrolyzable humic compounds in the soil. This leads to increased wheat yield and improved grain quality. Chemical and biochemical studies confirm the high agronomic efficiency of humic fertilizer Adept Agro.Bio.

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