The Effects of Physiologically Active Substances on Developing Plants and the Quality of Tomato Seeds

Fertilizers, physiologically active substances, and modern agricultural practices not only influence the yield in the year of sowing but also, to a certain extent, improve the hereditary and qualitative characteristics of the seed material in subsequent generations. The offspring of such plants are able to retain their characteristics for several years, even when transitioning to a standard diet.

The objective of our study was to investigate the effects of fertilizers containing physiologically active forms of humic acids in this context. The following solutions were tested: an organic substrate (as a modern analogue of humus), a specialized Totem Agro.Bio complex (as a highly effective activator of soil processes), and mineral analogues.

Experiments were conducted with the Kievskie 139 tomato variety over four years on ordinary black soil in the Dnipropetrovsk region with an average irrigation rate of 400-500 m³/ha. Seeds obtained from variously fertilized soils were sown in greenhouses. The resulting seedlings were then planted in the field under a uniform agronomic background.

There is a well-founded belief that physiologically active substances affect the rate of nucleic acid synthesis, influencing information transfer and protein metabolism, which determines key metabolic processes. Therefore, we conducted a cytophotometric determination of DNA quantity in the interphase nuclei of the root meristem of 6-day-old seedlings obtained from seeds whose maternal plants were grown in a fertilized environment.

RESULTS OF THE STUDY

The results of field experiments characterizing the effect of fertilizers on the yield of maternal tomato plants are presented in Table 1, and on the yield of first-generation plants - in Table 2. It was established that modern stimulants, especially in combination with preparations from the Agro.Bio line , provide a stable increase in productivity.

Table 1. Effect of some fertilizers on tomato yield (c/ha)

Table 2. The effect of fertilizers on the yield of first-generation tomato fruits (c/ha)

Analysis of first-generation seedlings revealed significant differences in biochemical composition. Use of the Totem Agro.Bio complex increased the average amount of DNA per nucleus compared to the control. Physiologically active forms of humic acids stimulate DNA synthesis in seedlings grown from seeds obtained from fertilized plots. The frequency of nuclei with higher DNA content was statistically significantly higher under the influence of these preparations.

The above material confirms that the active ingredients affect deep processes of seed formation. Their effect is not limited to immediate influence but continues into the next generation, which has important practical implications for vegetable seed production.

Agro.Bio Expert Note: Research shows that high-quality maternal plant nutrition, enriched with humic stimulants, creates a "genetic reserve" for future generations. Agro.Bio's modern Totem product , which combines humic acids with microbiological activity, enhances the vital potential of seeds, ensuring higher metabolic activity and yields.