Pathways of humate mobilization in soil and its effect on yields

It can now be considered a firmly established fact that soluble forms of humate acids have physiologically active properties, due to which their introduction into the soil increases the yield of a number of agricultural crops. However, there is every reason to believe that an agronomic effect can also be achieved by converting humic fulvic acids of the soil itself into a physiologically active state.

Since only when dissolved do humic and fulvic acids become capable of exhibiting their biologically active properties, which, in our opinion, is associated with their assimilation by the plant, we consider it possible to call this process mobilization.

It is well known that humic acids form soluble compounds with monovalent metals, and insoluble compounds with divalent and higher metals. Obviously, humates can be in forms of different solubility on different soils and, above all, depending on the composition of absorbed cations. In the case of calcium predominance in the composition of the absorbing complex, the solubility of humic acids will decrease, and in the presence of sodium, potassium and ammonium, it will increase. This should undoubtedly affect the vital activity of plants and microflora.

Experimental part

A shortened vegetation experiment was laid out in vessels with a capacity of 1 kg of soil (ordinary chernozem) in 3-fold repetition in two series according to the scheme: control, NaCl, NaHCO 3 , sodium humate, NH 4 OH and the same against the background of ammonium nitrate. The rate of these fertilizers, which we consider as a means of mobilizing soil humus, was set at the rate of 0.01 gram equivalent per 1 kg of soil. Ammonium nitrate was given at 0.14 g of nitrogen per vessel, which corresponds to the amount of nitrogen introduced with ammonia water.

Table 1. Dynamics of mobile humic substances in steaming vessels (in % of absolutely dry soil)

Experience options Humic acids soluble in 0.02n NaOH Water-soluble humus
May 29 June 7 September 10 May 29 June 7 September 10
Control 0.174 0.13 0.062 0.074 0,018 0,012
NaCl 0.220 0.15 0,090 0.073 0,017 0,014
NaHCO3 0.353 0.16 0.102 0.069 0,017 0,015
Sodium humate 0.138 0.16 0.095 0,120 0,019 0,011
NH4OH 0,190 0.15 0.138 0.076 0,021 0.006
NH4NO3 0.167 0.15 0.075 0,077 0.024 0,008
NH4NO3 + NaCl 0.285 0.16 0.061 0.064 0,020 0,012
NH4NO3 + NaHCO3 0.204 0.17 0.101 0.076 0,018 0,001
NH 4 NO 3 + sodium humate 0.131 0.15 6,090 0,071 0,020 0.004

Table 2. Dynamics of nitrates and ammonia in steaming vessels

Experience options Ammonia (mg per 100 g of soil) Nitrates (mg per 100 g of soil)
May 29 June 7 September 10 May 29 June 7 September 10
Control 5.10 2.71 2.84 18.56 12.48 6.79
NaCl 17.0 3.08 2.51 10.67 6.94 2.27
NaHCO3 14,14 3.07 2.87 16.87 13.84 6.13
Sodium humate 8.74 2.95 2.48 16.87 13.0 6.44
NH4OH 6.19 2.95 2.30 25.77 23.56 6.17
NH4NO3 4.28 2.73 2.53 27.12 30.43 10.89
NH4NO3 + NaCl 20.06 2.15 2.67 24.89 13.65 5.81
NH4NO3 + NaHCO3 14.87 2.28 2.51 27.12 25.05 4.47
NH 4 NO 3 + sodium humate 8.57 2.37 2.89 27.56 24.32 8.72

Table 3. Effect of some humus-dispersing agents on the dry matter weight of corn and the accumulation of ammonia, nitrates and mobile humic acids in the soil

Type of fertilizer Absolutely dry matter per vessel, g Ammonia (mg per 100 g of soil) Nitrates (mg per 100 g of soil) Humic substances soluble in
total including roots 0.02n NaOH H2O
Control - no fertilizer 13,14 5.17 2.69 8.37 0.14 0,018
NaCl 15.45 6.25 3.16 4.65 0.13 0.029
NaHCO3 13.78 5.57 3.06 10.31 0.16 0,018
Sodium humate 12.51 5.85 3.91 8.05 0.14 0,020
NH4OH 15.45 6.25 3.45 17.25 0.18 0,020
NH4NO3 14.28 5.14 2.28 19.84 0.10 0,019
NH4NO3 + NaCl 18.64 5.50 3.52 13.88 0.19 0.022
NH4NO3 + NaHCO3 14.28 5.09 2.28 18.29 0.16 0.023
NH 4 NO 3 + sodium humate 16:30 5.57 2.47 17.73 0.18 0,017

Table 4. Effect of fertilizing on the yield of stubble corn under irrigation (Based on field experiments)

Experience options 2015 2016 2017
Green mass yield of corn, c/ha Increase in centners/ha % increase Green mass yield of corn, c/ha Increase in centners/ha % increase Green mass yield of corn, c/ha Increase in centners/ha % increase
Control - no fertilizers 196 - - 425 - - 232 - -
Sodium humate top dressing 183 -13 -6.6 480 +55 +12.9 - - -
Top dressing N 20 P 20 (nitrogen in the form of NH 4 NO 3 ) 204 +8 +4.1 469 +44 +10.4 273 +41 +17.6
Top dressing N 20 P 20 + sodium humate (nitrogen in the form of NH 4 NO 3 ) 217 +21 +8.5 514 +89 +20.8 290 +58 +24.9
Top dressing N 20 P 20 (nitrogen in the form of NH 4 OH) 221 +25 +12.4 513 +88 +20.7 245 +13 +5.2

Table 5. Effect of fertilizers on the content of protein and sugars in green mass of corn (in % of dry matter)

Experience options 2016 2017
Protein Monosaccharides Sum of sugars Protein Monosaccharides Sum of sugars
Control (without fertilizers) 8.9 3.4 17.0 12.25 2.04 2.70
Sodium humate top dressing 8.0 5.0 22.0 - - -
Top dressing NH 4 NO 3 (N 20 in the background P 20 ) 9.9 4.6 23.0 12.25 2.42 2.95
Top dressing NH 4 NO 3 (N 20 in the background P 20 ) + sodium humate 9.3 4.3 21.5 12.5 2.15 3.15
Top dressing with NH4OH ( N20 in the background of P20 ) 9.1 4.0 20.8 9.5 1.92 2.35

CONCLUSIONS

  1. The introduction of humus-dispersing substances into the soil, such as sodium salts and ammonia water, increases the mobility of humic acids in the soil and converts them into a physiologically active state.
  2. The effect of these substances on the crop depends on the properties of the soil and meteorological conditions.
  3. The use of these substances on black soils in the presence of mineral food in the soil and under conditions when photosynthesis proceeds normally increases the yield of corn.

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